Journal: BMC Genomics
Article Title: Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
doi: 10.1186/1471-2164-9-266
Figure Lengend Snippet: GO-Slim analysis of annotated ESTs . The clustered EST data were annotated with terms from the GOSlim subset of the Gene Ontology system. For each term with more than eight clusters annotated across all libraries, we calculated the mean presence of that term per library, and the fold difference, compared to this mean, of the presence in each library. Panel A: GOSlim Function terms. Panel B: GOSlim Process terms. The acronyms for the cDNA libraries are: Ade, control adult earthworms; Lc, control late cocoon stage earthworms; Jv, control juvenile earthworms; Che, control adult earthworms, anterior segments; Cd, cadmium-exposed earthworms; Cu, copper-exposed earthworms; FLA, fluoranthene-exposed earthworms; ATZ, atrazine-exposed earthworms (for details, see Materials and Methods). For each GOslim term, the mean number of terms observed across all libraries is given in brackets. Some libraries had no annotations for particular GOslim terms (grouped as 'no annotations' on the figure).
Article Snippet: A suppression subtractive hybridisation library (Library 9) was also generated (using the Clontech PCR-Select cDNA subtraction method) to enrich for genes involved in reproduction with driver cDNA generated from posterior segments and tester cDNA from the segments containing the clitellum and seminal vesicles.
Techniques: Control